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Solved] What will be the optimal annealing temperatures for your first and... | Course Hero
Melting temperature (Tm°C) and annealing temperature (Ta°C) data... | Download Table
Computation of Melting Temperatures (Tm) of oligonucleotides for high throughput PCR using MS-Excel
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€